Our capabilities
Molecular Targets Program
Native Mass Spectrometry (MS) can observe native (folded) proteins complexed with ligands. We have identified protein-ligand complexes for > 100 proteins (malaria proteins, TB proteins, virus proteins and a series of miscellaneous proteins). The technology is robust including allowing the detection of both high and low affinity compounds. Lead discovery can be easily scaled to provide a massive parallel discovery platform. Lead ID requires protein purified by isolation or by cloning and a compound library. Protein can be chosen from among the 100 available proteins or be supplied. Compound libraries can be chosen from in-house libraries or be a provided library (synthetic or natural product derived).
- In-house Compound Library against partner Protein (Lead ID)
- Partner Compound Library against partner Protein (Lead ID)
- Partner Compound Library against in-house Protein (Lead ID)
- Compound against Partner Protein Panel (Target ID)
- Compound against in-house Protein Panel (Target ID)
- Extracts, Fractions, against partner Protein (Lead ID)
- Extracts, Fractions, against in-house Protein (Lead ID)
- Protein against Cell lysate (Metabolomics)
The human microbiome is attracting a lot of attention. MRMS can be used to pull-out the metabolite of any protein using the cell lysate of the organism that produced the protein. It is applicable to any culturable microorganisms or human cell line. Can explore the human microbiome (e.g. gut microbiome). Metabolomics requires protein purified by isolation or by cloning and a cell lysate from the organism that produces the protein.
Reference:
- Miaomiao Liu, Wesley C. Van Voorhis and Ronald J. Quinn*. Development of a target identification approach using native mass spectrometry. Sci. Rep. 2021, 11: 2387-2398
- Tin Mak, Jamie Rossjohn, Dene R. Littler, Miaomiao Liu*, and Ronald J. Quinn*. Collision-Induced Affinity Selection Mass Spectrometry for Identification of Ligands. ACS Bio Med Chem Au 2022
- Dene R. Littler*, Miaomiao Liu, Julie L. McAuley, Shea A. Lowery, Patricia T. Illing, Benjamin S. Gully, Anthony W. Purcell, Indu R. Chandrashekaran, Stanley Perlman, Damian F. J. Purcell, Ronald J. Quinn* and Jamie Rossjohn*. A natural product compound inhibits coronaviral replication in vitro by binding to the conserved Nsp9 SARS-CoV-2 protein. J. Biol. Chem. 2021, 297(6): 101362-101374
- Louise M Sternicki, Jim Nonomiya, Miaomiao Liu, Melinda M Mulvihill*, Ronald J Quinn*. Native Mass Spectrometry for the Study of PROTAC GNE-987-Containing Ternary Complexes. ChemMedChem. 2021, 16: 2206-2210
- Hoan Vu, Liliana Pedro, Tin Mak, Brendan McCormick, Jessica Rowley, Miaomiao Liu, Angela Di Capua, Billy Williams-Noonan, Ngoc B. Pham, Rebecca Pouwer, Bao Nguyen, Katherine T. Andrews, Tina Skinner-Adams, Jessica Kim, Wim G. J. Hol, Raymond Hui, Gregory J. Crowther, Wesley C. Van Voorhis and Ronald J. Quinn*. Fragment-based screening of a natural product library against 62 potential malaria drug targets employing native mass spectrometry. ACS Infect. Dis. 2018, 4 (4): 431–444