New publication
Identification of New Ligands for JNK3 by Fluorescence Thermal Shift Assays and Native Mass Spectrom
The group has performed a fluorescence thermal shift screening of a chemically diversified lead-like scaffold library of 2024 pure compounds and has identified seven JNK3 binding hits. We then used our unique native mass spectrometry platform to validate the interaction of 4 out of the 7 hits were found to bind JNK3. Binding geometries and interactions of the top 2 hits were evaluated by docking into a JNK3 crystal structure. Hit 5 had a Kd of 21 μM with JNK3 suggested scaffold 5-(phenylamino)-1H-1,2,3-triazole-4-carboxamide as a novel and selective JNK3 binder.
This work entitled "Identifying New Ligands for JNK3 by Fluorescence Thermal Shift Assays and Native Mass Spectrometry" has been published by ACS Omega. DOI: https://pubs.acs.org/doi/abs/10.1021/acsomega.2c00340
