New publication
Collision-Induced Affinity Selection Mass Spectrometry for Identification of Ligands
The group has developed a new MS-based strategy collision-induced affinity selection mass spectrometry (CIAS-MS), which allows separation and dissociation inside the instrument.
The quadrupole was used to select the ligand–protein complex and allow unbound molecules to be exhausted to vacuum. Collision-induced dissociation (CID) dissociated the protein–ligand complex, and the ion guide and resonance frequency were used to selectively detect the ligand. A known SARS-CoV-2 Nsp9 ligand, oridonin, was successfully detected when it was mixed with Nsp9. We provide proof-of-concept data that the CIAS-MS method can be used to identify binding ligands for any purified protein.
This work entitled "Collision-Induced Affinity Selection Mass Spectrometry for Identification of Ligands" has been published by ACS Bio Med Chem Au. DOI: https://pubs.acs.org/doi/abs/10.1021/acsbiomedchemau.2c00021
